Comparison of perinatal angiotensin binding in the brains of SHR and WKY rats by Victoria Irene Cook

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  • Hypertension -- Animal models.,
  • Angiotensins -- Physiological effect.,
  • Protein binding.

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Statementby Victoria Irene Cook.
The Physical Object
Paginationxii, 105 leaves, bound :
Number of Pages105
ID Numbers
Open LibraryOL16900355M

Download Comparison of perinatal angiotensin binding in the brains of SHR and WKY rats

Whole brain In addition to individual comparisons of each sep- arate brain area, which showed several differences between SHR and WKY during development; anal- yses of all brain regions at each different age also Comparisons of perinatal angiotensin binding in the forebrain of SHR and WKY rats MG TH PVN SFO MnPO LS OVLT 18th GD SHR WKY 21st GD Cited by: 4.

Components of the renin-angiotensin system have been identified in the rat fetus. Because of the association between the renin-angiotensin system and hypertension, we quantified angiotensin receptor binding sites in the brains of spontaneously hypertensive rats (SHR) and Wistar Kyoto (WKY) rats during perinatal by: 4.

Carlota O. Andrews, Joe W. Crim, Diane K. Hartle, Angiotensin II binding in area postrema and nucleus tractus solitarius of SHR and WKY rats, Brain Research Bulletin, /(93)T, 32, 4, (), ().Cited by: I[SI]-AII binding density was greatest in caudal AP and declined progressively in the rostral direction in both strains; however, binding density in SHR was significantly higher than in WKY rats at each level of the AP analyzed.

I[SI]-AII binding in the entire area postrema was approximately 46% higher in SHR rats. In the NTS, however Cited by:   On neonatal d 1 there was no significant difference in weight between the two strains, but by d 5 and 15 the SHR again had lower weights(p comparison to WKY. Figure 1 ( Cited by: The participation of main vasoactive systems (angiotensin II, norepinephrine, nitric oxide) in BP maintenance was investigated in conscious SHR and WKY rats.

I-Neuropeptide Y (I-NPY) binding capacities were quantitated in the areas postrema of young (5 weeks) and adult (20 week SHR and compared with age-matched WKY rats using autoradiography. Angiotensin was extracted from the brain ofadult rats, eachweighingg. The animals were bilaterally nephrecto-mized and brain tissue was obtained 24 hours later.

The brains were freed of blood by transcardiac perfusion with warmpercent saline containing 25 U ofheparinpermilliliter at 37°Candthen quickly removed, dissected, and frozen on.

Cook VI, Grove KL, Speth RC, McMenamin KM, Harding JW Differences between perinatal angiotensin binding in the brains of SHR and WKY rats.

Regul Pept – CAS. We evaluated if the brain bradykinin (BK) B1 receptor is involved in the regulation of blood pressure (BP) in conscious rats. Basal mean BP and HR were ±2 and ±3 mmHg and ±10 and ±14 be.

The SHR model was established by selective inbreeding of Wistar Kyoto rats (WKY). A total of 60 male SHR/Izm (Izumo) (10 weeks old, 20 mice/per experiment) rats were purchased from Central Lab, Animal Inc.

(Seoul, Korea). All animals were housed four per cage with food and water available ad libitum and maintained on a 12 h light/dark cycle. Amniotic fluid volume was found to be lower at days of gestation in the SHR compared with WKY rats, but continued to increase in the later stages of pregnancy, rather than diminish as was the case in the WKY, such that fluid volumes were significantly higher prior to term.

Each point is the mean of two determinations in duplicate. Specific binding of [ I]-angiotensin II to VSMC from WKY rats and SHR in the absence (−) and presence (+) of 10 −6M RNH B. Scatchard analysis of the binding characteristics of [ I]-angiotensin II to VSMC from WKY rats and SHR in the absence and presence of 10 −6M.

We studied the effect of propofol (– μmol/L; 1– μg/mL) on the mechanisms involved in Ca 2+ mobilization elicited by angiotensin II (AngII) in Wistar Kyoto (WKY) and spontaneously hypertensive (SHR) rats.

We studied the variations in intracellular Ca 2+ ([Ca 2+] i) concentrations in cultured aortic vascular smooth muscle cells (VSMCs) isolated from 6-wk-old WKY Comparison of perinatal angiotensin binding in the brains of SHR and WKY rats book SHR rats.

Three pairings of rats (two derived from divergent, selective breeding and one from divergent environmental conditions) were compared with regard to behavioral and hormonal parameters. Striking differences were observed: results obtained in our own laboratory as well as those found in a review of the literature pointed to higher emotionality (e.g., increased defecation and corticosterone.

In its classical view, the renin angiotensin system (RAS) was defined as an endocrine system involved in blood pressure regulation and body electrolyte balance.

However, the emerging concept of tissue RAS, along with the discovery of new RAS components, increased the physiological and clinical relevance of the system. Indeed, RAS has been shown to be expressed in various tissues where.

The following statistical levels were applied: p SHR and WKY rats; *, *** indicate differences (p rats of the same strain; i indicates differences (p SHR rats. Abstract. Receptors for angiotensin II (AII) were identified and characterized in testes of rats and several primate species.

Autoradiographic analysis of the binding of I-labeled (Sar1,Ile8)AII to rat, rhesus monkey, cebus monkey, and human testicular slide-mounted frozen sections indicated specific binding to Leydig cells in the interstitium. Products were identified by comparison of retention times to I-labeled standard peptides.

Schelling P. Increased sensitivity of neurons to angiotensin II in SHR as compared to WKY rats. Brain Res 63–69 Autoradiographic localization of subtypes of angiotensin II antagonist binding in the rat brain. Neuroscience – It has been proposed that angiotensin II interacts with the SNS to facilitate NE release in the kidney.

45 Renal denervation has been shown to delay the onset or decrease the severity of renal hypertension 46 47 and results in a major reduction in renal NE content in SHR and WKY. 47 Dihydrotestosterone also has been shown to have a tissue. Particularly, spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats were used to compare the effects of hypertension on focal cerebral ischemia.

All MCAo groups showed neurological deficiencies, motor dysfunction, and disruption of balancing ability; however, these pathological changes were more severe in SHR than WKY rats. As perinatal treatment with ATCE may partially correct the renal transcriptome in SHR, i.e., bring it closer to that of WKY, WKY (vs.

SHR), and SHR+ATCE (vs. SHR) were compared using Venn diagrams. At 2 days and 2 weeks, 18 and nine of the differentially expressed genes (about 11%) were in the same direction in WKY and SHR+ATCE respectively.

We evaluated if the brain bradykinin (BK) B 1 receptor is involved in the regulation of blood pressure (BP) in conscious rats.

Basal mean BP and HR were ±2 and ±3 mmHg and ±10 and ±14 beats min −1 in Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHR), respectively. Intracerebroventricular (i.c.v.) injection of 1 nmol B 1 receptor agonist Lys‐desArg 9 ‐BK.

The Brain Hepatocyte Growth Factor/c-Met Receptor System: A New Target for the Treatment of Alzheimer's Disease. Journal of Alzheimer's Disease: Jad. PMID DOI: /JAD Wright JW, Kawas LH, Harding JW. The renin-angiotensin system, both in the circulation and in the brain, is known for its role in the regulation of fluid balance and blood pressure.

The brain angiotensin II (Ang II) system is also involved in the control of anterior pituitary hormone secretion, through affecting the secretion of releasing and inhibitory factors into the hypophyseal portal vessels. Exposure of normotensive rats to angiotensin-converting enzyme (ACE) inhibitors in early life causes hypertrophy of intrarenal arteries.

Similar defects have been found in knockout mice lacking angiotensinogen, ACE, or angiotensin II type 1 (AT 1) the other hand, transient inhibition of the renin-angiotensin system from 2 weeks of age in spontaneously hypertensive rats (SHR.

SHR/y females have the parental WKY autosomes and X chromosomes and have no chromosomes of SHR origin; thus they are genetically equivalent to female WKY rats. One-way ANOVA showed significant blood pressure differences between WKY and SHR/y at 10 weeks and at 16 weeks (P.

20 Slaven B. Influence of salt and volume on changes in rat brain angiotensin. J Pharm Pharmacol. ; Crossref Medline Google Scholar; 21 Cole FE, Blakesley HL, Graci KA, Frohlich ED, MacPhee AA.

Brain angiotensin II receptor affinity and capacity in SHR and WKY rats: effects of acute dietary changes in NaCl. Brain Res. ; Angiotensin II type 1 receptor antagonists (ARBs) have become an important drug class in the treatment of hypertension and heart failure and the protection from diabetic nephropathy.

Eight ARBs are clinically available [azilsartan, candesartan, eprosartan, irbesartan, losartan, olmesartan, telmisartan, valsartan]. Azilsartan (in some countries), candesartan, and olmesartan are orally. Prehypertensive SHR from weeks of age have elevated renal inflammation and oxidative stress compared to age-matched WKY rats.

Renal artery stenosis results in reduced renal perfusion and pressure at the afferent arteriole, thus stimulating the release of. Conversely, in the aorta of 8-week-old rats, AT1 was the predominant angiotensin receptor subtype (71%).

In 8-week-old rats, the AT2 subtype was also present (28%). In pre- and postnatal rats, [I]Sar1-angiotensin II binding to AT1 receptors was sensitive to GTP gamma S whereas binding to AT2 receptors was not.

Spontaneously hypertensive rats (SHR) and the nonhypertensive Wistar-Kyoto (WKY) controls are widely used as a model for studying hypertension. The present study examined the renal gene expression profiles between SHR and WKY at a prehypertensive stage (3 wk of age) and hypertensive stage (9 wk of age).

Perinatal Exogenous Nitric Oxide in Fawn-Hooded Hypertensive Rats That Persistently Lowers Blood Pressure in Adult Life Reduces Renal Ribosomal Biogenesis in Early Life. Author URL. Wesseling S, Essers PB, Koeners MP, Pereboom TC, Braam B, van Faassen EE, Macinnes AW, Joles JA ().

ACE2 activity was significantly lower in hearts of day-old WKY rats and SHR compared with 3 mo of age. Although there was no difference in ACE2 activity between WKY rats and SHR at 10 days of age, there was a modest but significant decrease in cardiac ACE2 activity in 3-mo-old SHR compared with age-matched WKY rats.

Fig. Enter search terms. Keep search filters New search. Advanced search. Between 3 hours 15 minutes and 48 hours, angiotensin II or placebo could be adjusted to an infusion rate in milliliters per hour that was equivalent to a dose of to 40 ng per kilogram per. changes and affects brain GABA receptor bindings in spontaneously hypertensive (SHR) and Wistar Kyoto rats (WKY).

The Pharmacologist3. Tseng YT, Miyaoka T, Yu S, Ho IK. Region-specific alterations in [35S]TBPS binding sites of brain GABA A receptor after tolerance to and dependence upon pentobarbital. The Pharmacologist FELIX D., SCHELLING W. Increased sensitivity of neurons to angiotensin II in SHR as compared to WKY rats.

Brain Res. Crossref PubMed ISI Google Scholar; FELIX D., SCHLEGEL W. Angiotensin receptive neurones in the subfornical organ — structure-activity relations. Brain Res. Crossref PubMed ISI Google Scholar. 77 Figure 4 2 R epresentative 2D DIGE gel from PVN of SHR. Each boxed spot shows significantly up or down regulated protein in SHR compared to WKY.

PVN p rotein s were extracted from 3 WKY rats and SHR rats at 20 we eks in age A t least spo ts were obtained in each gel but only spots wer e matched in all three gels n=3. PAGE Moreover, in the group of WKY and SHR investigated in this study, the addition of a specific renin inhibitor to the preparation in no manner altered the production of angiotensins from Ang-().

These data showed that Ang-() is processed into the active angiotensin. Brains of spontaneously hypertensive (SH) rats express higher levels of angiotensin II, NET mRNA, and NE compared with WKY rats. Neuronal cultures (hypothalamus and brainstem) show a similar response to angiotensin II treatment, but the relative increase in NE uptake and NET mRNA expression is much larger than that observed in WKY rats.The physiological roles of ANG-(3–4) (Val-Tyr), a potent ANG II-derived peptide, remain largely unknown.

The present study 1)investigates whether ANG-(3–4) modulates ouabain-resistant Na+-ATPase re.Perinatal taurine depletion followed by high sugar intake (postweaning) alters the renin-angiotensin system (RAS) and glucose regulation in adult female rats.

This study tests the hypothesis that in adult female rats, RAS and estrogen contribute to insulin resistance resulting from perinatal .

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